MinION Mk1B (MN33054) Final report
— · LB4766 · AS32LB4766WT324 · MN33054
Protocol run ID: d6511aa6-61ba-44dc-89ef-3844d490d83d
DATA OUTPUT
Estimated bases
35.72 Mb
Reads generated
38.19 k
Estimated N50
1.15 kb
Total data produced (pass / fail)
1.79 GB
RUN DURATION
Run time
18 hrs 37 mins / 24 hrs 0 mins (est)
Elapsed time
Run limit
Run status
Stopped · By user
Flow cell type
FLO-FLG001
Flow cell ID
ASW732
Kit type
SQK-RNA002
Flongle adapter ID
FA-07358
Run limit
24 hrs
Active channel selection
On
Pore scan freq.
26 hrs
Reserved pores
Off
Basecalling
Off
Modified basecalling
Off
FAST5 output
vbz_compress
FAST5 reads per file
1000
FASTQ output
Off
POD5 output
Off
BAM output
Off
Bulk file output
Off
Data location
/var/lib/minknow/data/./LB4766/AS32LB4766WT324/20240320_1246_MN33054_ASW732_d6511aa6
MinKNOW
23.11.7
Bream
7.8.2
Configuration
5.8.6
Dorado
7.2.13
MinKNOW Core
5.8.6
The read length graph shows the total number of bases vs the read length. The longest 1% of strands are classified as outliers, and excluded to allow focus on the main body of data.
Legend
Basecalled
Estimated
The longest 1% of strands are classified as outliers, and aggregated into groups to show their relative amounts.
| Read length (kb) | Aggregated reads (kb) |
|---|---|
| 7 - 39 | 154.19 |
| 39 - 71 | 109.81 |
| 71 - 91 | 93.06 |
The cumulative output shows the total amount of bases or reads sequenced over time by your device.
Bases
Legend
Estimated
Predicted total number of bases, prior to basecalling
Reads
Legend
Total
Total number of reads, including passed, failed and skipped.
The Pore activity graph shows the performance of your sample as it is being sequenced during a run.
Legend
Sequencing
Pore currently sequencing
Adapter
Pore currently sequencing adapter
Pore available
Pore available for sequencing
Unavailable
Pore unavailable for sequencing
Active feedback
Channel ejecting analyte
No pore
No pore detected in channel
Out of range-high
Current is positive but unavailable for sequencing
Out of range-low
Current is negative but unavailable for sequencing
Multiple
Multiple pores detected. Unavailable for sequencing.
Saturated
The channel has switched off as current levels exceed hardware limitations
Zero
Pore currently unavailable for sequencing
Channel disabled
Channel is disabled and awaiting another pore scan
Unclassified
Pore status unknown
Sequencing
Pore currently sequencing
Pore available
Pore available for sequencing
Unavailable
Pore currently unavailable for sequencing
Inactive
Pore no longer suitable for further sequencing
Unclassified
Pore status unknown
General
Some commonly seen issues are excess pores classified as Recovering, Open Pore, or Free Adapter. To find out what advice is applicable for your run, visit the user guide.
A Pore scan is performed at configurable time intervals to determine the current status of pores within channels on a Flow Cell. For this run a Pore scan is performed every 26 hrs.
Legend
Pore available
Pore in channel available for sequencing
Reserved pore
Pore in reserve, will return to available when required
Unavailable
Pore inhibited from sequencing
Saturated
Possible contamination in the sample
Zero
No current is passing through this pore, possibly due to bubbles on the membrane
Inactive
Pore no longer suitable for further sequencing
High proportion Unavailable
Possible contaminants in library blocking the pore. Consider using the Flow Cell Wash Kit, and reloading a library.
High proportion Inactive
If localised to one area of the Flow Cell, this could indicate that an air bubble has been introduced during the flushing/loading steps. If inactivity is spread across the Flow Cell this could be caused by improper loading of the library, please refer to the user guide for further support.
The temperature of the Flow Cell over the run time.
Legend
Measured
Target
Out of range
Check that the Flow Cell is correctly seated and firmly pushed down into the device. Ensure ambient temperature is always within the specified range for your device in the user guide.
Air flow should be good but not excessive. Excessive amounts of cool air blowing on the device could prevent it from reaching target temperature.
SYSTEM MESSAGES
System messages are a record of the events that occurred in the time covered by this report.
Byte
B
Kilobyte
KB
Megabyte
MB
Gigabyte
GB
Terabyte
TB
Base
b
Kilobase
kb
Megabase
Mb
Gigabase
Gb
Terabase
Tb
Minutes
mins
Hours
hrs